Cover & TOC
Document type
články
články
Document record
Source: BMČ - články
Title
Heterogeneity of Human Pancreatic Islet Isolation Around Europe: Results of a Survey Study / R. Nano, JA. Kerr-Conte, H. Scholz, M. Engelse, M. Karlsson, F. Saudek, D. Bosco, B. Antonioli, F. Bertuzzi, PRV. Johnson, B. Ludwing, Z. Ling, DL. De Paep, B. Keymeulen, F. Pattou, T. Berney, O. Korsgren, E. de Koning, L. Piemonti,
Author
Nano, Rita
Diabetes Research Institute, IRCCS San Raffaele Scientific Institute, Milan, Italy.

Kerr-Conte, Julie A
European Genomic Institute for Diabetes, University Hospital Lille, Lille, France.

Scholz, Hanne
Department of Transplant Medicine, Oslo University Hospital, Oslo, Norway.

Engelse, Marten
Department of Nephrology, Leiden University Medical Center, Leiden, Netherlands.

Karlsson, Marie
Uppsala University Hospital, Uppsala, Sweden.

Saudek, Frantisek
Diabetes Center, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.

Bosco, Domenico
Hôpitaux Universitaires de Genève, Genève, Switzerland.

Antonioli, Barbara
Diabetology Unit, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy.

Bertuzzi, Federico
Diabetology Unit, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy.

Johnson, Paul R V
Islet Transplant Research Group, Nuffield Department of Surgical Sciences and Oxford Centre for Diabetes, University of Oxford, Oxford, United Kingdom.

Ludwing, Barbara
Department of Medicine III, University Hospital Carl Gustav Carus Dresden, Dresden, Germany.

Ling, Zhidong
Academic Hospital and Diabetes Research Center, Vrije Universiteit Brussel, Brussels, Belgium.

De Paep, Diedert L
Academic Hospital and Diabetes Research Center, Vrije Universiteit Brussel, Brussels, Belgium.

Keymeulen, Bart
Academic Hospital and Diabetes Research Center, Vrije Universiteit Brussel, Brussels, Belgium.

Pattou, François
European Genomic Institute for Diabetes, University Hospital Lille, Lille, France.

Berney, Thierry
Hôpitaux Universitaires de Genève, Genève, Switzerland.

Korsgren, Olle
Uppsala University Hospital, Uppsala, Sweden.

de Koning, Eelco
Department of Nephrology, Leiden University Medical Center, Leiden, Netherlands.

Piemonti, Lorenzo
Diabetes Research Institute, IRCCS San Raffaele Scientific Institute, Milan, Italy. Vita-Salute San Raffaele University, Milan, Italy.

Cited source
Transplantation. 2020, roč. 104, č. 1, s. 190-196. ISSN: 0041-1337; 1534-6080 (elektronická verze).
Date of issue
2020
Language
angličtina
Country
Spojené státy americké
Document type
články
DOI
Pubmed ID
Link
Record number
bmc20028690
Persistent link
MESH descriptor
mladiství
dospělí
věkové faktory
staří
počet buněk (normy, statistika a číselné údaje)
separace buněk (metody, statistika a číselné údaje)
buňky kultivované (transplantace)
dítě
předškolní dítě
Evropa
lidé
kojenec
novorozenec
Langerhansovy ostrůvky (cytologie)
lidé středního věku
perfuze (metody, statistika a číselné údaje)
průzkumy a dotazníky (statistika a číselné údaje)
časové faktory
transplantace Langerhansových ostrůvků (metody, normy)
směrnice pro lékařskou praxi jako téma
odběr tkání a orgánů (metody, normy, statistika a číselné údaje)
výběr dárců (metody, normy, statistika a číselné údaje)
studená ischemie (normy, statistika a číselné údaje)
mladý dospělý
primární buněčná kultura (metody, normy, statistika a číselné údaje)
Genre
English Abstract
BACKGROUND: Europe is currently the most active region in the field of pancreatic islet transplantation, and many of the leading groups are actually achieving similar good outcomes. Further collaborative advances in the field require the standardization of islet cell product isolation processes, and this work aimed to identify differences in the human pancreatic islet isolation processes within European countries. METHODS: A web-based questionnaire about critical steps, including donor selection, pancreas processing, pancreas perfusion and digestion, islet counting and culture, islet quality evaluation, microbiological evaluation, and release criteria of the product, was completed by isolation facilities participating at the Ninth International European Pancreas and Islet Transplant Association (EPITA) Workshop on Islet-Beta Cell Replacement in Milan. RESULTS: Eleven islet isolation facilities completed the questionnaire. The facilities reported 445 and 53 islet isolations per year over the last 3 years from deceased organ donors and pancreatectomized patients, respectively. This activity resulted in 120 and 40 infusions per year in allograft and autograft recipients, respectively. Differences among facilities emerged in donor selection (age, cold ischemia time, intensive care unit length, amylase concentration), pancreas procurement, isolation procedures (brand and concentration of collagenase, additive, maximum acceptable digestion time), quality evaluation, and release criteria for transplantation (glucose-stimulated insulin secretion tests, islet numbers, and purity). Moreover, even when a high concordance about the relevance of one parameter was evident, thresholds for the acceptance were different among facilities. CONCLUSIONS: The result highlighted the presence of a heterogeneity in the islet cell product process and product release criteria.
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