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Document type
články
články
Document record
Source: BMČ - články
Title
Evaluation of endothelial glycocalyx in healthy volunteers - An observational study / D. Astapenko, A. Ticha, A. Tomasova, R. Hyspler, Z. Zadak, C. Lehmann, V. Cerny,
Author
Astapenko, David
Department of Anaesthesiology and Intensive Care Medicine, University Hospital Hradec Kralove, Czech Republic. Charles University, Faculty of Medicine in Hradec Kralove, Hradec Kralove, Czech Republic. Centrum for Research and Development, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic.

Tichá, Alena
Centrum for Research and Development, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic. Department of Clinical Biochemistry and Diagnostics, University Hospital Hradec Kralove, Czech Republic.

Tomasova, Adela
Department of Clinical Biochemistry and Diagnostics, University Hospital Hradec Kralove, Czech Republic.

Hyspler, Radomír
Charles University, Faculty of Medicine in Hradec Kralove, Hradec Kralove, Czech Republic. Centrum for Research and Development, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic. Department of Clinical Biochemistry and Diagnostics, University Hospital Hradec Kralove, Czech Republic.

Zadák, Zdeněk
Charles University, Faculty of Medicine in Hradec Kralove, Hradec Kralove, Czech Republic. Centrum for Research and Development, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic.

Lehmann, Christian
Department of Anaesthesia, Pain Management and Perioperative Medicine, Dalhousie University, Halifax, NS, Canada. Department of Microbiology and Immunology, Dalhousie University, Halifax, NS, Canada. Department of Pharmacology, Dalhousie University, Halifax, NS, Canada. Department of Physiology and Biophysics, Dalhousie University, Halifax, NS, Canada.

Cerny, Vladimir
Department of Anaesthesiology and Intensive Care Medicine, University Hospital Hradec Kralove, Czech Republic. Charles University, Faculty of Medicine in Hradec Kralove, Hradec Kralove, Czech Republic. Centrum for Research and Development, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic. Department of Anaesthesia, Pain Management and Perioperative Medicine, Dalhousie University, Halifax, NS, Canada. Department of Anaesthesiology, Perioperative Medicine and Intensive Care, Jan Evangelista Purkinje University, Masaryk Hospital, Usti nad Labem, Czech Republic.

Cited source
Clinical hemorheology and microcirculation. 2020, roč. 75, č. 3, s. 257-265. ISSN: 1386-0291; 1875-8622 (online).
Date of issue
2020
Language
angličtina
Country
Nizozemsko
Document type
články
DOI
Pubmed ID
Link
Record number
bmc20028624
Persistent link
English Abstract
BACKGROUND: Endothelial glycocalyx (EG) is a carbohydrate-rich gel-like mesh covering the apical surface of endothelial cells. It has been linked to the microvascular pathophysiology and tissue metabolism. However, little is known about its condition in young healthy adults. OBJECTIVE: We aimed to describe the condition of EG in young healthy adults by in vivo EG imaging and measurement of syndecan-1, a plasma marker of EG integrity in order to obtain reference values. METHODS: For in vivo EG studies we used Side-stream Dark Field imaging of the sublingual microcirculation. Recordings were analysed automatically by GlycoCheck software providing the Perfused Boundary Region (PBR) as a marker of EG thickness. Levels of syndecan-1 were analysed in plasma samples by ELISA. RESULTS: 21 volunteers were included in the study. Median of the PBR value was 1.82 μm (interquartile range 1.69-2.01, 95% CI 1.79-1.97). Median concentration of syndecan-1 was 0.3 ng/ml (interquartile range 0.23-0.39, 95% CI 0.27-0.49). CONCLUSION: This study provides a comparison for cohorts of patients with a particular disease where the EG is presumably damaged. Our findings do not entirely comply with already published data in healthy individuals.
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