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články
články
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Source: BMČ - články
Title
Controlled Peptide-Mediated Vesicle Fusion Assessed by Simultaneous Dual-Colour Time-Lapsed Fluorescence Microscopy / NL. Mora, AL. Boyle, BJV. Kolck, A. Rossen, Š. Pokorná, A. Koukalová, R. Šachl, M. Hof, A. Kros,
Author
Mora, Nestor Lopez
Supramolecular and Biomaterials Chemistry, Leiden Institute of Chemistry, Leiden University, P.O. Box 9502, 2300, RA, Leiden, The Netherlands.

Boyle, Aimee L
Supramolecular and Biomaterials Chemistry, Leiden Institute of Chemistry, Leiden University, P.O. Box 9502, 2300, RA, Leiden, The Netherlands. Macromolecular Biochemistry, Leiden Institute of Chemistry, Leiden University, P.O. Box 9502, 2300 RA, Leiden, The Netherlands.

Kolck, Bart Jan van
Supramolecular and Biomaterials Chemistry, Leiden Institute of Chemistry, Leiden University, P.O. Box 9502, 2300, RA, Leiden, The Netherlands.

Rossen, Anouk
Supramolecular and Biomaterials Chemistry, Leiden Institute of Chemistry, Leiden University, P.O. Box 9502, 2300, RA, Leiden, The Netherlands.

Pokorná, Šárka
J. Heyrovský Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, v.v.i., Dolejškova 2155/3, 182 23, Prague, 8, Czech Republic.

Koukalova, Alena
J. Heyrovský Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, v.v.i., Dolejškova 2155/3, 182 23, Prague, 8, Czech Republic.

Šachl, Radek
J. Heyrovský Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, v.v.i., Dolejškova 2155/3, 182 23, Prague, 8, Czech Republic.

Hof, Martin
J. Heyrovský Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, v.v.i., Dolejškova 2155/3, 182 23, Prague, 8, Czech Republic. martin.hof@jh-inst.cas.cz.

Kros, Alexander
Supramolecular and Biomaterials Chemistry, Leiden Institute of Chemistry, Leiden University, P.O. Box 9502, 2300, RA, Leiden, The Netherlands. a.kros@chem.leidenuniv.nl.

Cited source
Scientific reports. 2020, roč. 10, č. 1, s. 3087. ISSN: 2045-2322; (OCoLC)732869387.
Date of issue
2020
Language
angličtina
Country
Spojené království
Document type
články
DOI
Pubmed ID
Link
Record number
bmc20028489
Persistent link
English Abstract
We have employed a model system, inspired by SNARE proteins, to facilitate membrane fusion between Giant Unilamellar Vesicles (GUVs) and Large Unilamellar Vesicles (LUVs) under physiological conditions. In this system, two synthetic lipopeptide constructs comprising the coiled-coil heterodimer-forming peptides K4, (KIAALKE)4, or E4, (EIAALEK)4, a PEG spacer of variable length, and a cholesterol moiety to anchor the peptides into the liposome membrane replace the natural SNARE proteins. GUVs are functionalized with one of the lipopeptide constructs and the fusion process is triggered by adding LUVs bearing the complementary lipopeptide. Dual-colour time lapse fluorescence microscopy was used to visualize lipid- and content-mixing. Using conventional confocal microscopy, lipid mixing was observed on the lipid bilayer of individual GUVs. In addition to lipid-mixing, content-mixing assays showed a low efficiency due to clustering of K4-functionalized LUVs on the GUVs target membranes. We showed that, through the use of the non-ionic surfactant Tween 20, content-mixing between GUVs and LUVs could be improved, meaning this system has the potential to be employed for drug delivery in biological systems.
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