Cover & TOC
Document type
články
články
Document record
Source: BMČ - články
Title
A new mixed inhibitor of adenosine deaminase produced by endophytic Cochliobolus sp. from medicinal plant seeds / XG. Zhang, QY. Sun, P. Tang, GY. Ma, GJ. Guo, SJ. Guo, XD. Ma,
Author
Zhang, Xin-Guo
School of Life Science and Engineering, Key Laboratory of Herbal-Tebitan Drug Screening and Deep Processing of Gansu Province, Lanzhou University of Technology, Lanzhou, 730050, China. biodrug@163.com.

Sun, Qiao-Yun
School of Life Science and Engineering, Key Laboratory of Herbal-Tebitan Drug Screening and Deep Processing of Gansu Province, Lanzhou University of Technology, Lanzhou, 730050, China.

Tang, Peng
School of Life Science and Engineering, Key Laboratory of Herbal-Tebitan Drug Screening and Deep Processing of Gansu Province, Lanzhou University of Technology, Lanzhou, 730050, China.

Ma, Guo-Yan
School of Life Science and Engineering, Key Laboratory of Herbal-Tebitan Drug Screening and Deep Processing of Gansu Province, Lanzhou University of Technology, Lanzhou, 730050, China.

Guo, Guang-Jun
School of Life Science and Engineering, Key Laboratory of Herbal-Tebitan Drug Screening and Deep Processing of Gansu Province, Lanzhou University of Technology, Lanzhou, 730050, China.

Guo, Si-Jia
School of Life Science and Engineering, Key Laboratory of Herbal-Tebitan Drug Screening and Deep Processing of Gansu Province, Lanzhou University of Technology, Lanzhou, 730050, China.

Ma, Xiao-Di
School of Life Science and Engineering, Key Laboratory of Herbal-Tebitan Drug Screening and Deep Processing of Gansu Province, Lanzhou University of Technology, Lanzhou, 730050, China.

Cited source
Folia microbiologica (Prague). 2020, roč. 65, č. 2, s. 293-302. ISSN: 0015-5632 (tištěná verze); 1874-9356 (online verze).
Date of issue
2020
Language
angličtina
Country
Spojené státy americké
Document type
články
DOI
Grant number
31860004 (National Natural Science Foundation of China)     vyhledat publikace
17JR5RA128 (Gansu province natural science fund)     vyhledat publikace
20180802 (Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research, Ministry of Education (CN)     vyhledat publikace
Pubmed ID
Link
Record number
bmc20024266
Persistent link
MESH descriptor
adenosindeaminasa (metabolismus, chemie)
Ascomycota (klasifikace, genetika, izolace a purifikace, chemie)
vazebná místa
lidé
nádory (farmakoterapie, enzymologie)
magnetická rezonanční spektroskopie
léčivé rostliny (mikrobiologie)
semena rostlinná (mikrobiologie)
inhibitory adenosindeaminasy (farmakologie, chemie)
endofyty (klasifikace, genetika, izolace a purifikace, chemie)
molekulový docking
Genre
English Abstract
Medicinal plants have been studied for potential endophytic interactions and numerous studies have provided evidence that seeds harbor diverse microbial communities, not only on their surfaces but also within the embryo. Adenosine deaminase (ADA) is known as a potential therapeutic target for the treatment of lymphoproliferative disorders and cancer. Therefore, in this study, 20 types of medicinal plant seeds were used to screen endophytic fungi with tissue homogenate and streak. In addition, 128 morphologically distinct endophyte strains were isolated and their ADA inhibitory activity determined by a spectrophotometric assay. The strain with the highest inhibitory activity was identified as Cochliobolus sp. Seven compounds were isolated from the strain using a chromatography method. Compound 3 showed the highest ADA inhibitory activity and was identified as 5-hydroxy-2-hydroxymethyl-4H-pyran-4-one, based on the results of 1H and 13C NMR spectroscopy. The results of molecular docking suggested that compound 3 binds to the active site and the nonspecific binding site of the ADA. Furthermore, we found that compound 3 is a mixed ADA inhibitor. These results indicate that endophytic strains are a promising source of ADA inhibitors and that compound 3 may be a superior source for use in the preparation of biologically active ADA inhibitor compounds used to treat cancer.
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